Cat No.
NATE-0285
Description
Geranylgeranyltransferase type 1 or simply geranylgeranyltransferase is one of the three enzymes in the prenyltransferase group. In specific terms, Geranylgeranyltransferase (GGTase 1) adds a 20-carbon isoprenoid called a geranylgeranyl group to proteins bearing a CaaX motif:a four-amino acid sequence at the carboxyl terminal of a protein. Geranylgeranyltransferase inhibitors are being investigated as anti-cancer agents.
Abbr
GGTase, Recombinant (Rat)
Alias
GGTase-I; GGTase
Source
baculovirus infected insect cells
Species
Rat
Form
Solution
Bio-activity
> 200 units/mg protein
CAS No.
160141-09-3
Molecular Mass
mol wt 43 kDa (β subunit); mol wt 48 kDa (α subunit)
Purification
>80% (SDS-PAGE)
Unit Definition
One unit will transfer one nanomole of geranylgeranyl isoprenoid to H-Ras (CVLL isoform) per hour at pH 8.0 at 37°C.
Stability
?70°C
Preparation Instructions
Host cells were coinfected with the α subunit of farnesyl transferase and the β subunit of geranylgeranyltransferase I.
Gene Name
Pggt1b protein geranylgeranyltransferase type I, beta subunit [Rattus norvegicus]
Synonyms
geranylgeranyltransferase type I; GGTase-I; GGTase; 160141-09-3; Geranylgeranyltransferase
GeneID
81746
mRNA Refseq
NM_031082
Protein Refseq
NP_112344
Function
Contributes_to CAAX-protein geranylgeranyltransferase activity; CAAX-protein geranylgeranyltransferase activity; Contributes_to drug binding; Contributes_to isoprenoid binding; metal ion binding; Contributes_to peptide binding; prenyltransferase activity; protein binding; transferase activity; Contributes_to zinc ion binding
