Cat No.
NATE-1155
Description
The activity of glycerol kinase is found widely in nature. In microorganisms GK makes possible the utilization of glycerol as a carbon source. In mammals the enzyme represents a juncture of sugar and fat metabolism; The enzyme is important to the clinical chemist in the determination of glycerol. GK is also useful in the assay of glyceraldehydes and dihydroxyacetone following their quantitative reduction to glycerol with sodium borohydride.
Abbr
GK, Native (Flavobacterium meningosepticum)
Alias
GK
Species
Flavobacterium meningosepticum
Form
Freeze dried powder
Enzyme Commission Number
EC 2.7.1.30
Bio-activity
More than 70 U/mg solid
Appearance
White to light grayish white amorphous powder, lyophilized.
Molecular Mass
150 kDa (TSK G3000SWXL)
50 kDa (SDS?PAGE)
pH Stability
5.0?11.0
Michaelis Constant
Glycerol 8.8 × 10-5M
ATP 3.0 × 10-5M
Isoelectric point
4.3
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μmole of glycerol to glycerol?3?phosphate per minute at 37°C under the conditions specified in the assay procedure.
Optimum pH
8
Optimum temperature
80°C
Thermal stability
Stable at 60°C and below
Applications
Useful for the measurement of Triglyceride.
Synonyms
glycerokinase; GK; ATP: glycerol-3-phosphotransferase; glycerol kinase phosphorylating; glyceric kinase; EC 2.7.1.30
