Cat No.
NATE-1094
Description
Aspartate transaminase (AST) or aspartate aminotransferase, also known as AspAT/ASAT/AAT or serum glutamic oxaloacetic transaminase (SGOT), is a pyridoxal phosphate (PLP)-dependent transaminase enzyme (EC 2.6.1.1). AST catalyzes the reversible transfer of an α-amino group between aspartate and glutamate and, as such, is an important enzyme in amino acid metabolism. AST is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells. Serum AST level, serum ALT (alanine transaminase) level, and their ratio (AST/ALT ratio) are commonly measured clinically as biomarkers for liver health. The tests are part of blood panels.
Abbr
GOT, Recombinant (E. coli)
Alias
GOT
Species
E. coli
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 2.6.1.1
Bio-activity
180 U/mg protein at pH 8.5 and 25°C.
Molecular Mass
~ 45,737
Isoelectric point
~ 6.1
Unit Definition
One Unit of L-glutamic-oxaloacetic transaminase (GOT) is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH.
Optimum pH
8.5
Thermal stability
up to 25°C
Stability
> 4 years at 4°C
Preparation Instructions
For assay, this enzyme should be diluted in 3 μM pyridoxal phosphate containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use.
Applications
High purity Glutamate oxaloacetate transaminase (E. coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
EC 2.6.1.1; glutamic-oxaloacetic transaminase; glutamic-aspartic transaminase; transaminase A; AAT; AspT; 2-oxoglutaRate-glutamate aminotransferase; aspartate α-ketoglutaRate transaminase; aspartate aminotransferase; aspartate-2-oxoglutaRate transaminase; aspartic acid aminotransferase; aspartic aminotransferase; aspartyl aminotransferase; AST; glutamate-oxalacetate aminotransferase; glutamate-oxalate transaminase; glutamic-aspartic aminotransferase; glutamic-oxalacetic transaminase; glutamic oxalic transaminase; GOT (enzyme); L-aspartate transaminase; L-aspartate-α-ketoglutaRate transaminase; L-aspartate-2-ketoglutaRate aminotransferase; L-aspartate-2-oxoglutaRate aminotransferase; L-aspartate-2-oxoglutaRate-transaminase; L-aspartic aminotransferase; oxaloacetate-aspartate aminotransferase; oxaloacetate transferase; aspartate:2-oxoglutaRate aminotransferase; glutamate oxaloacetate transaminase; 9000-97-9
