Cat No.
NATE-1157
Description
A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinase can transfer an inorganic phosphate group from ATP to a substrate. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose.
Abbr
Hexokinase, Native (Bacillus sp.)
Species
Bacillus sp.
Form
Freeze dried powder
Enzyme Commission Number
EC 2.7.1.1
Bio-activity
More than 250 U/mg solid
Appearance
White amorphous powder, lyophilized
Molecular Mass
68 kDa (gel filtration)
pH Stability
7.0?8.5
Michaelis Constant
Glucose 8.2 × 10-4M
ATP 8.7 × 10-5M
MgCl2 1.6 × 10-3M
Isoelectric point
5.64
Unit Definition
One unit is defined as the amount of enzyme which generates 1 μmole of NADPH per minute at 37oC under the conditions specified in the assay procedure.
Optimum pH
7.5?8.0
Optimum temperature
50°C
Stabilizers
ATP, albumin, KCl, NaCl
Thermal stability
Stable at 55°C and below
Applications
This enzyme is useful for enzymatic determination of glucose or creatinine kinase activity when coupled with glucose?6?phosphate dehydrogenase.
Synonyms
hexokinase (phosphorylating); ATP-dependent hexokinase; glucose ATP phosphotransferase; hexokinase; ATP:D-hexose 6-phosphotransferase; EC 2.7.1.1
