Cat No.
NATE-1156
Description
A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinase can transfer an inorganic phosphate group from ATP to a substrate. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose.
Abbr
Hexokinase, Native (Rhodothermus obamensis)
Species
Rhodothermus obamensis
Form
Freeze dried powder
Enzyme Commission Number
EC 2.7.1.1
Bio-activity
100 - 400 U/mg
Appearance
White to light grayish lyophilized powder.
Molecular Mass
140 kDa (gel filtration)
pH Stability
5～10
Michaelis Constant
Glucose 0.46 mM
ATP 0.21 mM
Unit Definition
One unit is defined as the amount of enzyme which generates 1 μmole of NADPH per minute at 37oC under the conditions specified in the assay procedure
Optimum pH
7.5?8.0
Thermal stability
Stable at 55°C and below
Applications
This enzyme is useful for enzymatic determination of glucose or creatinine kinase activity when coupled with glucose?6?phosphate dehydrogenase.
Synonyms
hexokinase (phosphorylating); ATP-dependent hexokinase; glucose ATP phosphotransferase; hexokinase; ATP:D-hexose 6-phosphotransferase; EC 2.7.1.1
