Cat No.
NATE-1097
Description
A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinase can transfer an inorganic phosphate group from ATP to a substrate. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose.
Abbr
Hexokinase, Native (Yeast)
Species
Yeast
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 2.7.1.1
Bio-activity
98 U/mg protein at pH 7.4 and 25°C.
Molecular Mass
~ 53,738
Isoelectric point
~ 5.3
Unit Definition
One Unit of hexokinase is defined as the amount of enzyme required to produce one μmole of NADH from NAD+.
Optimum pH
7.6
Thermal stability
up to 40°C
Stability
> 4 years at 4°C
Preparation Instructions
For assay, this enzyme should be diluted in 100 mM Tris.HCl buffer, pH 7.6 containing 5 mM MgCl2 and 0.5 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use.
Applications
High purity Hexokinase (yeast) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
hexokinase (phosphorylating); ATP-dependent hexokinase; glucose ATP phosphotransferase; hexokinase; ATP:D-hexose 6-phosphotransferase; EC 2.7.1.1
