Cat No.
NATE-0989
Description
A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinase can transfer an inorganic phosphate group from ATP to a substrate. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose.
Abbr
HK, chemically modified (Yeast)
Species
Yeast
Bio-activity
> 40 U/mg lyophilizate
Appearance
White lyophilizate
Molecular Mass
57 kD (SDS-PAGE)
pH Stability
5.0-9.0
Michaelis Constant
Phosphate buffer, 0.1 mol/l, pH 7.0; +25°C: 3.05 x 10-4 mol/l
Phosphate buffer, 0.1 mol/l, pH 7.4; +30°C: 1.90 x 10-4 mol/l
Tea buffer, 0.1 mol/l, pH 7.6; +25°C: 2.30 x 10-4 mol/l
Isoelectric point
4.5-5.0
Specificity
Hexokinase phosphorylates D-glucose, D-fructose, D-mannose, D-glucosamin, 2-deoxyglucose. L-Arabinose, D-xylose, L-rhamnose, D-galactose, D-lactose, sucrose, maltose, trehalose, raffinose, N-acetyl glucosamine do not react. ATP can be partially replaced by other nucleotides.
Optimum pH
7.0-10.0
Activators
Mg2+, catecholamines
Stability
At +2 to +8°C within specification range for 18 months. Store dry.
Inhibitors
EDTA, SH-blocking compounds, sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxymethylglucose, lyxose.
Applications
Use Hexokinase in diagnostic tests for blood glucose using the hexokinase method and for the determination of creatine kinase.
Synonyms
hexokinase type IV glucokinase; hexokinase D; hexokinase type IV; hexokinase (phosphorylating); ATP-dependent hexokinase; glucose ATP phosphotransferase; hexokinase; ATP:D-hexose 6-phosphotransferase
