Cat No.
NATE-1135
Description
In enzymology, a ADP-Dependent Hexokinase (EC 2.7.1.147) is an enzyme that catalyzes the chemical reaction: D?Glucose + ADP → D?Glucose?6?phosphate + AMP.
Abbr
Hexokinase (ADP-Dependent), Native (Pyrococcus furiosus)
Species
Pyrococcus furiosus
Enzyme Commission Number
EC 2.7.1.147
Bio-activity
More than 30 U/mg solid
Appearance
White amorphous powder, lyophilized
Molecular Mass
100 kDa (gel filtration)
51 kDa (SDS?PAGE)
pH Stability
5.0?10.0
Michaelis Constant
Glucose 0.64 mM (at 37°C)
ADP 0.07 mM (at 37°C)
Isoelectric point
5.1
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μmole of glucose to D?Glucose?6?phosphate per minute at 37°C under the conditions specified in the assay procedure.
Optimum pH
7.5
Optimum temperature
100°C
Activators
Mg2+, Co2+, Mn2+
Thermal stability
Stable at 95°C and below
Applications
Useful for the enzymatic determination of ADP.
Synonyms
ADP-dependent glucokinase; ADP-specific glucokinase; ADP:D-glucose 6-phosphotransferase; EC 2.7.1.147
