Cat No.
NATE-0551
Description
Phosphofructokinase is a kinase enzyme that phosphorylates fructose 6-phosphate in glycolysis. The enzyme-catalysed transfer of a phosphoryl group from ATP is an important reaction in a wide variety of biological processes. One enzyme that utilizes this reaction is phosphofructokinase (PFK), which catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate, a key regulatory step in the glycolytic pathway. PFK exists as a homotetramer in bacteria and mammals (where each monomer possesses 2 similar domains) and as an octomer in yeast (where there are 4 alpha-(PFK1) and 4 beta-chains (PFK2), the latter, like the mammalian monomers, possessing 2 similar domains[3]). This protein may use the morpheein model of allosteric regulation.
Abbr
PFK, Native (Bacillus stearothermophilus)
Alias
PFKWII; PFK
Species
Bacillus stearothermophilus
Form
Freeze dried powder
Enzyme Commission Number
EC 2.7.1.11
Bio-activity
> 250 U/mg
Appearance
White to pale yellow powder
Molecular Mass
72 kDa (gel filtration); 35 kDa (SDS?PAGE)
pH Stability
6.0-10.0 (37°C, 60 mins)
Michaelis Constant
D?Fructose?6?phosphate (D?F?6?P) 5.8 mM (at 37°C); ATP 0.07 mM (at 37°C)
Isoelectric point
pH 5.9
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μmole of fructose?6?phosphate to Fructose?1,6 ?bisphosphate per minute at 37°C under the conditions specified in the assay procedure.
Optimum pH
9
Activators
Mg2+
Thermal stability
Stable at 55°C and below (pH 8.5, 30 mins)
Applications
Useful for enzymatic determiantion of fructose-6-phosphate
Synonyms
PFKWII; EC 2.7.1.11; PFK; phosphofructokinase; 6-phosphofructokinase; Phosphofructokinase I; Phosphohexokinase
