Cat No.
NATE-0562
Description
Phosphorylase A is the active form of glycogen phosphorylase which converts glycogen and orthophosphate (Pi) to glucose 1-phoshate (G-1-P). Phosphorylase A can be inhibited by these compounds:Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls. Dimeric phosphorylase b is converted to the more active tetramer, phosphorylase a, by the action of phosphorylase kinase.
Abbr
Phosphorylase a, Native (Rabbit)
Source
Rabbit muscle
Species
Rabbit
Form
Lyophilized powder containing β-glycerophosphate and EDTA
Enzyme Commission Number
EC 2.4.1.1
Bio-activity
20-30 units/mg protein
CAS No.
9032-10-4
Purification
2× crystallization
Unit Definition
One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate per min at pH 6.8 at 30°C, measured in a system containing phosphoglucomutase, NADP, and glucose-6-phosphate dehydrogenase. (One μmolar unit is equivalent to ~45 Cori units.)
Applications
Phosphorylase from rabbit muscle has been used in a study to assess the molecular mechanisms of oleanolic acid. It has also been used in a study to describe the conversion of phosphorylase B to A, through a conversion enzyme in the presence of 32P-ATP.
Synonyms
Phosphorylase a; EC 2.4.1.1; 9032-10-4; muscle phosphorylase a and b; amylophosphorylase; polyphosphorylase; amylopectin phosphorylase; glucan phosphorylase; α-glucan phosphorylase; 1,4-α-glucan phosphorylase; glucosan phosphorylase; granulose phosphorylase; maltodextrin phosphorylase; muscle phosphorylase; myophosphorylase; potato phosphorylase; starch phosphorylase; 1,4-α-D-glucan:phosphate α-D-glucosyltransferase; phosphorylase (ambiguous)
