Cat No.
NATE-0575
Description
Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Abbr
PKCE, Recombinant (Human)
Alias
PRKCE; PKCE
Source
baculovirus infected insect cells
Species
Human
Form
buffered aqueous glycerol solution
Enzyme Commission Number
EC 2.7.1.37
Molecular Mass
apparent mol wt 89-96 kDa
Purification
>95% (SDS-PAGE)
Unit Definition
One unit will transfer 1 nmol of phosphate to PKC ε substrate peptide in 1 min at pH 7.4 at 30°C.
Gene Name
PRKCE protein kinase C, epsilon [Homo sapiens]
Synonyms
PRKCE; protein kinase C, epsilon; protein kinase C epsilon type; PKCE; nPKC-epsilon; Ca2+-activated phospholipid-dependent serine-threonine kinase, ε isozyme human; PKCε human; PKCε; EC 2.7.1.37
GeneID
5581
mRNA Refseq
NM_005400
Protein Refseq
NP_005391
MIM
176975
UniProt ID
Q02156
Chromosome Location
2p21
Pathway
B Cell Receptor Signaling Pathway, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; DAG and IP3 signaling, organism-specific biosystem; Disease, organism-specific biosystem; Downstream signal transduction, organism-specific biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem
Function
ATP binding; SH3 domain binding; actin monomer binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; ethanol binding; metal ion binding; nucleotide binding; protein kinase C activity; protein kinase binding; receptor activator activity; signal transducer activity
