Cat No.
NATE-0574
Description
Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Abbr
PKCA, Recombinant (Human)
Alias
PRKCA; PKCA; PKCα
Source
baculovirus infected insect cells
Species
Human
Form
buffered aqueous glycerol solution
Molecular Mass
mol wt 80-81 kDa by SDS-PAGE
Purification
> 70% (SDS-PAGE)
Unit Definition
One unit will transfer 1 pmol of phosphate to CREBtide in 1 min at pH 7.2 at 30°C
Stability
?70°C
Gene Name
PRKCA protein kinase C, alpha [Homo sapiens]
Synonyms
PRKCA; protein kinase C, alpha; PKCA; protein kinase C alpha type; PKC-A; PKCα; AAG6; PKC-alpha; PRKACA
GeneID
5578
mRNA Refseq
NM_002737
Protein Refseq
NP_002728
MIM
176960
UniProt ID
P17252
Chromosome Location
17q22-q24
Pathway
ATF-2 transcription factor network, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Amoebiasis, organism-specific biosystem
Function
ATP binding; enzyme binding; histone kinase activity (H3-T6 specific); metal ion binding; nucleotide binding; protein binding; protein kinase C activity; protein kinase activity; protein kinase activity; protein serine/threonine kinase activity; zinc ion binding
