Cat No.
NATE-0625
Description
Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Abbr
PRKCZ, Recombinant (Human)
Alias
PRKCZ; PKC2
Source
Baculovirus infected insect cells
Species
Human
Form
buffered aqueous solution; Solution in 20 mM HEPES, pH 7.5; 2 mM EDTA, 2 mM EGTA, 5 mM DTT, 250 mM NaCl, 0.05% Triton X-100, and 50% glycerol.
Enzyme Commission Number
EC 2.7.1.37
Molecular Mass
mol wt 76-80 kDa by SDS-PAGE
Purification
> 75% (SDS-PAGE)
Gene Name
PRKCZ protein kinase C, zeta [Homo sapiens]
Synonyms
PRKCZ; protein kinase C, zeta; protein kinase C zeta type; PKC2; PKC-ZETA; EC 2.7.1.37
GeneID
5590
mRNA Refseq
NM_001033581
Protein Refseq
NP_001028753
MIM
176982
UniProt ID
Q05513
Chromosome Location
1p36.33-p36.2
Pathway
CDC42 signaling events, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Ceramide signaling pathway, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; EGFR1 Signaling Pathway, organism-specific biosystem
Function
ATP binding; insulin receptor substrate binding; metal ion binding; nucleotide binding; protein binding; protein kinase C activity; protein kinase activity; protein serine/threonine kinase activity; zinc ion binding
