Cat No.
NATE-0580
Description
Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.
Abbr
PKG1B, Recombinant (Human)
Alias
PRKG1B; PRKGR1B; PKG1B
Source
baculovirus infected insect cells
Species
Human
Form
buffered aqueous glycerol solution
Bio-activity
> 1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))
Molecular Mass
mol wt 76 kDa (monomer)
Purification
>95% (SDS-PAGE)
Unit Definition
One unit will phosphorylate 1 micromole of VASPtide (RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.
Stability
?20°C
Applications
Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.
Gene Name
PRKG1 protein kinase, cGMP-dependent, type I [Homo sapiens]
Synonyms
Protein Kinase G Iβ; PRKG1B; PRKGR1B; PKG1B; cGMP-dependent protein kinase 1; cGKI-BETA
GeneID
5592
mRNA Refseq
NM_001098512
Protein Refseq
NP_001091982
MIM
176894
UniProt ID
Q13976
Chromosome Location
10q11.2
Pathway
Adaptive Immune System, organism-specific biosystem; Gap junction, organism-specific biosystem; Gap junction, conserved biosystem; Hemostasis, organism-specific biosystem; Immune System, organism-specific biosystem; Long-term depression, organism-specific biosystem; Long-term depression, conserved biosystem
Function
ATP binding; cGMP binding; cGMP-dependent protein kinase activity; calcium channel regulator activity; nucleotide binding; protein binding; protein serine/threonine kinase activity
