Cat No.
NATE-0605
Description
Polynucleotide kinase catalyses a "forward reaction" transfer of the γ-phosphate of ATP to the 5' hydroxyl terminus of single-and double-stranded nucleic acids (DNA and RNA) and 3'-nucleoside monophosphates. In exchange reactions containing ADP, the enzyme will catalyze the exchange of 5'-terminal phosphate groups and ATP. The 3'-phosphatase activity enables the enzyme to remove 3'-phosphoryl groups from phosphorylpolynucleotides.
Abbr
PNK, Native (T4-infected Escherichia coli)
Alias
PNK; DNA kinase
Species
T4-infected Escherichia coli
Form
buffered aqueous glycerol solution
Enzyme Commission Number
EC 2.7.1.78
Bio-activity
10 units/μL
CAS No.
37211-65-7
Concentration
10 units/μL
Molecular Mass
mol wt 33 kDa
Unit Definition
One unit catalyzes the transfer of one nanomole of 32P to the 5'-end of micrococcal nuclease-treated DNA in 30 min. at 37°C. Transfer is detected as incorporation into acid-insoluble material.
Applications
Suitable for: ? Sequencing or nucleic acid tagging (DNA and RNA) by 5′-end labeling ? 5′ phosphorylation of oligonucleotides ? Removal of 3′-phosphate groups from phosphorylpolynucleotides
Warnings
Activity is determined in a reaction mixture containing 40 mM Tris-HCl (pH 7.5), with 10 mM MgCl2, 5 mM dithiothreitol, 0.5 mM 5’-OH polynucleotide ends, and mM [γ-32P]-ATP.
Synonyms
polynucleotide 5'-hydroxyl-kinase; EC 2.7.1.78; 37211-65-7; ATP:5'-dephosphopolynucleotide 5'-phosphatase; PNK; polynucleotide 5'-hydroxyl kinase (phosphorylating); 5'-hydroxyl polynucleotide kinase; 5'-hydroxyl polyribonucleotide kinase; 5'-hydroxyl RNA kinase; DNA 5'-hydroxyl kinase; DNA kinase; polynucleotide kinase; polynucleotide 5'-hydroxy-kinase
