Cat No.
NATE-0871
Description
Phenylethanolamine N-methyltransferase (PNMT) is the enzyme which catalyzes the N-methylation of norepinephrine thereby resulting in the formation of epinephrine as shown below: Norepinephrine + S-Adenosyl methionine (SAM) -------> Epinephrine. The mechanism involves transfer of an active methyl group from S-adenosylmethionine (SAM) to the primary amino group of norepinephrine. Although it is primarily localized in the adrenal medulla, PNMT activity has also been demonstrated in the brain and heart tissues of several mammalian species including humans. PNMT purified from ox, rat and rabbit adrenal medulla have molecular weights in the range of 37,000-38,000. Analysis of PNMT activity could provide valuable information in the evaluation of catecholamine metabolism.
Abbr
PNMT, Native (Bovine)
Alias
PNMT
Source
Bovine Adrenal Medulla
Species
Bovine
Form
Freeze-dried powder
Enzyme Commission Number
EC 2.1.1.28
Bio-activity
50-100 U/mg protein
Molecular Mass
37-38 kDa
Unit Definition
The amount of enzyme which will convert one nanomole of normetanephrine to metanephrine per hour at pH 8.5 at 37°C.
Stability
Store at -20°C (-4°F)
Synonyms
phenylethanolamine N-methyltransferase; noradrenaline N-methyltransferase; noradrenalin N-methyltransferase; norepinephrine methyltransferase; norepinephrine N-methyltransferase; phenethanolamine methyltransferase; phenethanolamine N-methyltransferase; Phenylethanolamine N-Methyl Transferase; PNMT; S-adenosyl-L-methionine:phenylethanolamine-N-methyltransferase; EC 2.1.1.28
