Cat No.
NATE-1120
Description
Phosphotransacetylase converts CoA to acetyl CoA. This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. This enzyme participates in 3 metabolic pathways:taurine and hypotaurine metabolism, pyruvate metabolism, and propanoate metabolism.
Abbr
PTA, Recombinant (B. subtilis)
Alias
PTA
Species
Bacillus subtilis
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 2.3.1.8
Bio-activity
13027 U/mg protein at pH 7.4 and 25°C.
Molecular Mass
~ 36,953
Isoelectric point
~ 5.3
Unit Definition
One Unit of phosphotransacetylase is the amount of enzyme required to produce one μmole of acetyl-CoA from coenzyme A per minute measured at 233 nm.
Optimum pH
7.4
Thermal stability
up to 25°C
Stability
> 2 years at 4°C
Preparation Instructions
For assay, this enzyme should be diluted in 100 mM Tris.HCl buffer, pH 7.4 containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use.
Applications
High purity recombinant Phosphotransacetylase (B. subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This enzyme is commonly used, in conjunction with acetate kinase, for the measurement of acetic acid.
Synonyms
phosphate acetyltransferase; phosphotransacetylase; phosphoacylase; PTA; EC 2.3.1.8; 9029-91-8
