Cat No.
NATE-0660
Description
This Reverse Transcriptase has exceptionally strong strand displacement activity and enables efficient preparation of cDNA up to 12 kb in length. It is robust, versatile and well-suited for applications requiring full-length cDNA such as preparation of cDNA libraries and other techniques involving first strand cDNA synthesis (RT-PCR, preparation of cDNA probes, real-time quantitative RT-PCR). It can be used for performing a reverse transcription reaction with any RNA template including GC-rich templates and RNAs with high levels of secondary structure. This enzyme is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase and is verified to be RNase H Minus. Because of the excellent extension capability of PrimeScript Reverse Transcriptase, preparation of cDNA can be performed at a lower temperature (42°C), decreasing the risk of RNA degradation that can occur during conventional reactions performed at higher temperatures.
Abbr
RT, Recombinant (Moloney Murine Leukemia Virus)
Alias
RT
Source
E. coli
Species
Moloney Murine Leukemia Virus
Unit Definition
One unit is the amount of the enzyme that incorporates 1 nmol of [3H]dTTP in 10 minutes at 37°C, with poly (rA), oligo (dT) 12-18 as the primer-template.
Applications
RT-PCR; First strand cDNA synthesis; cDNA probe preparation; Synthesis of cDNA libraries with a high proportion of full-length cDNAs.
Synonyms
Reverse transcriptase; RT
