Cat No.
NATE-0669
Description
Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages. The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad. The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.
Abbr
SGE, Native (Leuconostoc mesenteroides)
Alias
SGE; CEP
Species
Leuconostoc mesenteroides
Form
Lyophilized powder containing dextran, MES buffer salts and CaCl2
Enzyme Commission Number
EC 2.4.1.5
Bio-activity
> 100 units/mg protein
CAS No.
9032-14-8
Unit Definition
One unit will liberate 1.0 μmole of fructose per min at 37°C, pH 5.4.
Applications
Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases. The enzyme from Creative Enzymes has been used to prepare immobilized sphere for the production of dextran from sucrose.
Synonyms
EC 2.4.1.5, sucrose 6-glucosyltransferase; SGE; CEP; sucrose-1,6-α-glucan glucosyltransferase; sucrose:1,6-α-D-glucan 6-α-D-glucosyltransferase; 9032-14-8
