Cat No.
NATE-0692
Description
Bovine terminal transferase (TdT) is a primer-dependent polymerase that catalyzes the addition of deoxynucleotides to the 3'-OH terminus of DNA molecules with the release of inorganic phosphate. TdT reacts preferentially with either single-stranded DNA molecules or double-stranded-DNA with 3' overhangs, but procedures have been developed to label blunt ends or 3'-recessive ends. In a reaction mixture, the divalent ion (Co2+, Mn2+, Mg2+) will influence purine and pyrimidine polymerization rate. Activities of TdT are also affected by the bases (dATP, dCTP, dGTP and dTTP) present.
Abbr
TdT, Native (Calf)
Alias
TdT
Source
Calf thymus
Species
Calf
Form
buffered aqueous glycerol solution
Enzyme Commission Number
EC 2.7.7.31
CAS No.
9027-67-2
Concentration
>5000 U/mL
Molecular Mass
mol wt 60 kDa
Unit Definition
One unit will incorporate 1 nanomole of dATP into acid-precipitable material in one hour at 37°C using d (pT)6 as primer.
Applications
Suitable for: ? Addition of homopolymers to vectors, inserts and cDNA for cloning ? Labeling the 3′-end of double-and single-stranded DNA with non-radioactive or radioactive labels ? Carrying out in vitro mutagenesis by adding single nucleotides to DNA ? Use in TUNEL assays
Warnings
Activity assay uses 200 mM potassium cacodylate, pH 7.2, 4 mM MgCl2, 1 mM 2-mercaptoethanol, 1 mM 3H-dATP, 70 μM d (pT)6, 37°C.
Gene Name
DNTT DNA nucleotidylexotransferase [Bos taurus]
Synonyms
DNA nucleotidylexotransferase; terminal deoxyribonucleotidyltransferase; terminal addition enzyme; addase; deoxynucleotidyl terminal transferase; deoxyribonucleic acid nucleotidyltransferase; deoxyribonucleic nucleotidyltransferase; terminal deoxynucleotide transferase; TdT; EC 2.7.7.31; 9027-67-2
GeneID
281120
mRNA Refseq
NM_177495
Protein Refseq
NP_803461
UniProt ID
P06526
Chromosome Location
chromosome: 26
Function
DNA binding; DNA nucleotidylexotransferase activity; DNA-directed DNA polymerase activity
