Cat No.
NATE-0738
Description
ZAP-70 is protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells. It is required for T cell activation. It is activated by Lck-mediated phosphorylation of its tyrosine residues. ZAP-70 Tyrosine Kinase is a protein belongs to Syk family and is essential for T cell activation. It facilitates the upregulation of Fas ligand in activation-induced T cell apoptosis. Additionally, it also plays a pivotal role in LFA-1-dependent T Cell Migration.
Abbr
ZAP70, Recombinant (Human)
Alias
ZAP70; SRK; TZK; ZAP-70; STD
Source
baculovirus infected insect cells (Histidine tagged)
Species
Human
Form
buffered aqueous glycerol solution
Bio-activity
> 150 U/mg
Molecular Mass
mol wt 70 kDa
Purification
> 90% (SDS-PAGE)
Unit Definition
One unit will phosphorylate 1 nmol polyglutamic acid:tyrosine per min at 37°C at pH 7.0.
Stability
?70°C
Applications
Human ZAP-70 Tyrosine Kinase was used for mapping the phosphorylation sites on LAT (linker for activation of T cells) for investigating the mechanism of activation of signalling proteins in T cells.
Gene Name
ZAP70 zeta-chain (TCR) associated protein kinase 70kDa [Homo sapiens]
Synonyms
ZAP-70 Tyrosine Kinase; Zeta-chain-associated protein kinase 70; ZAP-70; SRK; STCD; STD; TZK; ZAP70
GeneID
7535
mRNA Refseq
NM_001079
Protein Refseq
NP_001070
UniProt ID
P43403
Chromosome Location
2q11-q13
Pathway
Adaptive Immune System, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Generation of second messenger molecules, organism-specific biosystem; Immune System, organism-specific biosystem; Inflammatory Response Pathway, organism-specific biosystem; Natural killer cell mediated cytotoxicity, organism-specific biosystem; Natural killer cell mediated cytotoxicity, conserved biosystem
Function
ATP binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity
