Cat No.
NATE-0748
Description
Chymotrypsinogen is a proteolytic enzyme and a precursor (zymogen) of the digestive enzyme chymotrypsin. It is a single polypetide chain consisting of 245 amino acid residues. It is synthesized in the acinar cells of the pancreas and stored inside membrane-bounded granules at the apex of the acinar cell. The cell is then stimulated by either a hormonal signal or a nerve impulse and the contents of the granules spill into a duct leading into the duodenum.
Abbr
α-Chymotrypsinogen A, Native (Bovine)
Source
Bovine Pancreas
Species
Bovine
Form
essentially salt-free, lyophilized powder
Bio-activity
> 40 units/mg solid
CAS No.
9035-75-0
Unit Definition
After activation to Chymotrypsin, one unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25°C.
Applications
The enzyme has been used in the non-invasive determination of solid-state protein conformation using near infrared (NIR) spectroscopy. It has been used to study the partitioning of protein in polymer/polymer aqueous two-phase systems. The enzyme has also been used for self-interaction chromatography applications, to test the rapid measurement of protein osmotic second virial coefficients. In this technique, the protein is immobilized on chromatographic particles and its retention is measured using isocratic elution.
Gene Name
LOC618826 chymotrypsinogen B1-like [Bos taurus]
Synonyms
9035-75-0; Chymotrypsinogen; α-Chymotrypsinogen A; Chymotrypsinogen A; Chymotrypsin
GeneID
618826
mRNA Refseq
NM_001105454
Protein Refseq
NP_001098924
Chromosome Location
chromosome: 18
Function
serine-type endopeptidase activity
