Cat No.
NATE-1086
Description
Glycoside hydrolases (also called glycosidases or glycosyl hydrolases) assist in the hydrolysis of glycosidic bonds in complex sugars. They are extremely common enzymes with roles in nature including degradation of biomass such as cellulose and hemicellulose, in anti-bacterial defense strategies (e.g., lysozyme), in pathogenesis mechanisms (e.g., viral neuraminidases) and in normal cellular function (e.g., trimming mannosidases involved in N-linked glycoprotein biosynthesis). Together with glycosyltransferases, glycosidases form the major catalytic machinery for the synthesis and breakage of glycosidic bonds.
Abbr
α-Glucosidase, Native (B. stearothermophilus)
Species
Bacillus stearothermophilus
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.20
Bio-activity
> 80 U/mg
Concentration
750 Units/mL
Molecular Mass
57750
pH Stability
5.0 - 11.0
Isoelectric point
5.3 and 5.5
Unit Definition
One Unit of enzyme activity is the amount of enzyme required to release one μmole of p-nitrophenol per min from the appropriate substrate at pH 6.5 and 40°C.
Optimum pH
6.0-7.0
Optimum temperature
60°C
Thermal stability
< 60°C
Stability
> 4 years at 4°C
Preparation Instructions
We recommend the inclusion of BSA (0.5 mg/mL) in all buffers.
Applications
High purity alpha-Glucosidase (B. stearothermophilus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
Alpha-glucosidase; EC 3.2.1.20; maltase; glucoinvertase; glucosidosucrase; maltase-glucoamylase; alpha-glucopyranosidase; glucosidoinvertase; alpha-D-glucosidase; alpha-glucoside hydrolase; alpha-1,4-glucosidase; alpha-D-glucoside glucohydrolase; glycosidases; glycosyl hydrolases; α-Glucosidase
