Cat No.
NATE-1085
Description
Glycoside hydrolases (also called glycosidases or glycosyl hydrolases) assist in the hydrolysis of glycosidic bonds in complex sugars. They are extremely common enzymes with roles in nature including degradation of biomass such as cellulose and hemicellulose, in anti-bacterial defense strategies (e.g., lysozyme), in pathogenesis mechanisms (e.g., viral neuraminidases) and in normal cellular function (e.g., trimming mannosidases involved in N-linked glycoprotein biosynthesis). Together with glycosyltransferases, glycosidases form the major catalytic machinery for the synthesis and breakage of glycosidic bonds.
Abbr
α-Glucosidase, Recombinant (Thermotoga maritima)
Species
Thermotoga maritima
Form
A solution containing 50% glycerol plus 0.02% (w/v) sodium azide
Enzyme Commission Number
EC 3.2.1.20
Bio-activity
32 U/mg protein (on p-NP-α-D-glucopyranoside) at pH 7.5 and 80°C.
~ 11 U/mg protein (on p-NP-α-D-glucopyranoside) at pH 7.5 and 60°C.
CAS No.
9001-42-7
Molecular Mass
~ 56,000
pH Stability
4.0 - 9.0
Isoelectric point
6.1
Specificity
Hydrolysis of terminal, non-reducing (1,4)-α-linked D-glucose residues with release of α-Dglucose.
Unit Definition
One Unit of α-glucosidase activity is defined as the amount of enzyme required to release one μmole of of p-nitrophenol (p-NP) per minute from p-nitrophenyl-α-D-glucopyranoside (5 mM) in Tris.HCl buffer (100 mM), pH 7.5 at 80°C.
Optimum pH
7.5 - 8.5
Optimum temperature
80 - 100°C
Thermal stability
up to 80°C
Stability
> 4 years at -20°C
Preparation Instructions
For assay, this enzyme should be diluted in Tris.HCl buffer (100 mM), pH 7.5 containing 1 mg/mL BSA, 3 mM NAD, 4 mM manganese chloride and 400 mM mercaptoethanol. Swirl to mix the enzyme immediately prior to use.
Applications
Potential applications in the biofuels, biotechnology and carbohydrate research industries.
Synonyms
Alpha-glucosidase; EC 3.2.1.20; maltase; glucoinvertase; glucosidosucrase; maltase-glucoamylase; alpha-glucopyranosidase; glucosidoinvertase; alpha-D-glucosidase; alpha-glucoside hydrolase; alpha-1,4-glucosidase; alpha-D-glucoside glucohydrolase; glycosidases; glycosyl hydrolases; α-Glucosidase
