Cat No.
NATE-1093
Description
In enzymology, an alpha-glucuronidase (EC 3.2.1.139) is an enzyme that catalyzes the chemical reaction: an alpha-D-glucuronoside + H2O ? an alcohol + D-glucuronate. Thus, the two substrates of this enzyme are alpha-D-glucuronoside and H2O, whereas its two products are alcohol and D-glucuronate. This enzyme belongs to the family of hydrolases, to be specific those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is alpha-D-glucosiduronate glucuronohydrolase. This enzyme is also called alpha-glucosiduronase.
Abbr
α-Glucuronidase, Recombinant (Geobacillus stearothermophilus)
Species
Geobacillus stearothermophilus
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.139
Bio-activity
7.4 U/mg protein (on Aldouronic acid mixture; tri:tetra:penta) at pH 7.0 and 70°C.
Molecular Mass
~ 93,200
pH Stability
6.0 - 9.0
Isoelectric point
~ 5.4
Specificity
Hydrolysis of the α-1,2 glycosidic bond between D-glucuronic acid or its ether 4-O-methyl-D-glucuronic acid and D-xylose residues of xylo-oligosaccharides (aldo-uronic acids) from xylan.
Unit Definition
One Unit of α-D-glucuronidase activity is defined as the amount of enzyme required to release one μmole of α-D-glucuronic acid per minute from aldouronic acid (tri:tetra:penta) in MOPS buffer (100 mM) pH 7.0 and 70°C.
Optimum pH
7
Optimum temperature
70°C
Thermal stability
up to 70°C
Stability
> 2 years at 4°C
Preparation Instructions
For assay, this enzyme should be diluted in MOPS buffer (100 mM), pH 7.0 containing 0.5 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant alpha-D-Glucuronidase (Geobacillus stearothermophilus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
EC 3.2.1.139; alpha-D-glucosiduronate glucuronohydrolase; alpha-glucosiduronase
