Cat No.
NATE-1047
Description
Alpha-N-arabinofuranosidase is an enzyme with system name alpha-L-arabinofuranoside arabinofuranohydrolase. This enzyme catalyses the following chemical reaction: Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides. The enzyme acts on alpha-L-arabinofuranosides, alpha-L-arabinans containing (1,3)- and/or (1,5)-linkages, arabinoxylans and arabinogalactans.
Abbr
α-L-Arabinofuranosidase, Recombinant (Clostridium thermocellum)
Species
Clostridium thermocellum
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.55
Bio-activity
58 U/mg protein (on p-nitrophenyl-α-L-arabinofuranoside) at pH 5.5 and 40°C; ~155 U/mg protein at pH 5.5 and 60°C.
CAS No.
9067-74-7
Molecular Mass
~ 58,500
pH Stability
5.0 - 9.0
Isoelectric point
~ 5.7
Unit Definition
One Unit of α-L-arabinfuranosidase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol (p-NP) per minute from p-nitrophenyl-α-L-arabinofuranoside (2.5 mM) in sodium acetate buffer (100 mM).
Optimum pH
5.0 - 6.0
Optimum temperature
60°C
Thermal stability
up to 60°C
Stability
> 4 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 5.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant alpha-L-Arabinofuranosidase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase; EC 3.2.1.55; arabinosidase; alpha-arabinosidase; alpha-L-arabinosidase; alpha-arabinofuranosidase; polysaccharide alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside hydrolase; L-arabinosidase; alpha-L-arabinanase; Alpha-N-arabinofuranosidase; α-L-Arabinofuranosidase
