Cat No.
NATE-0931
Description
α-N-acetylgalactosaminidase (EC 3.2.1.49) is a glycoside hydrolase from bacteria and animals, also known as nagalase. The human gene that codes for this enzyme is NAGA. Mutations in this gene and the deficiency in alpha-N-acetylgalactosaminidase activity have been identified as the cause of Schindler disease.
Abbr
α-NAGA, Recombinant (Microbial)
Alias
α-NAGAL; α-NAGA; α-GalNAcase
Species
Microbial
Form
In solution (Tris.HCl/NaCl/EDTA)
Enzyme Commission Number
EC 3.2.1.49
Bio-activity
15.0 U/mg protein (on pNP-α-D-N-acetylgalactosamine) at pH 7.5 and 37°C
Molecular Mass
~ 88,900 Da
pH Stability
4.5 - 8.0
Isoelectric point
~ 5.3
Specificity
Hydrolysis of terminal non-reducing N-acetyl-D-galactosamine residues in N-acetyl-α-D-galactosaminides from glycoproteins and oligosaccharides.
Unit Definition
One Unit of α-N-acetyl-D-galactosaminidase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from pNP-α-D-Nacetylgalactosamine (1 mM) in Tris.HCl buffer (10 mM) pH 7.5 and 37°C, monitored at 410 nm.
Optimum pH
6.0 - 8.0
Optimum temperature
37°C
Preparation Instructions
For assay, this enzyme should be diluted in Tris.HCl buffer (10 mM), pH 7.0 containing 1 mg/ml BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant alpha-N-Acetylgalactosaminidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
EC 3.2.1.49; α-N-acetylgalactosaminidase; Alpha-N-acetylgalactosaminidase; α-acetylgalactosaminidase; N-acetyl-α-D-galactosaminidase; N-acetyl-α-galactosaminidase; α-NAGAL; α-NAGA; α-GalNAcase
