Cat No.
NATE-0972
Description
Releases α(2-3,6)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates.
Abbr
α-Neuraminidase, Recombinant (Clostridium perfringens)
Alias
Sialidase
Source
E. coli
Species
Clostridium perfringens
Form
A sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).
Bio-activity
>10 U/ml (>40 U/mg)
Molecular Mass
~41 kD
Specificity
This enzyme releases α2-3, and α2-6 linked N-acetylneuraminic acid from complex carbohydrates. This enzyme will not efficiently cleave NeuAc α2-6 linked to N-acetylgalactosamine (GalNAc) when the GalNAc is labeled with a fluorophore.
Unit Definition
One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37°C, pH 5.5.
Optimum pH
6
Applications
Structural analysis of oligosaccharides; Determining sialic acid linkage; Glycoprotein deglycosylation; Removing heterogeneity from
glycoproteins
Synonyms
neuraminidase; sialidase; α-neuraminidase; acetylneuraminidase; exo-α-sialidase
