Cat No.
NATE-0932
Description
Alpha-D-xyloside xylohydrolase (EC 3.2.1.177, alpha-xylosidase) is an enzyme. This enzyme catalyses the following chemical reaction: Hydrolysis of terminal, non-reducing alpha-D-xylose residues with release of alpha-D-xylose. The enzyme catalyses hydrolysis of a terminal, unsubstituted xyloside at the extreme reducing end of a xylogluco-oligosaccharide.
Abbr
α-Xylosidase, Recombinant (E. coli)
Species
Escherichia coli
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.177
Bio-activity
1.8 U/mg protein (on isoprimeverose) at pH 7.0 and 50°C.
CAS No.
53362-86-0
Molecular Mass
~ 88.9 kDa
pH Stability
4.0-10.0 (> 75% control activity after 24 h at 4°C)
Isoelectric point
~ 5.7
Specificity
Hydrolysis of terminal, non-reducing alpha-D-xylose residues with release of alpha-D-xylose.
Unit Definition
One Unit of α-xylosidase activity is defined as the amount of enzyme required to release one μmole of of xylose per min from isoprimeverose (10 mg/mL) in glycylglycine buffer (100 mM), pH 7.0 at 50°C.
Optimum pH
7
Optimum temperature
50°C
Stability
> 4 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in glycylglycine (100 mM), pH 7.0 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity α-Xylosidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
Alpha-D-xyloside xylohydrolase; EC 3.2.1.177; alpha-xylosidase; α-Xylosidase
