Cat No.
NATE-1075
Description
β-galactosidase is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins.
Abbr
GLB, Native (Aspergillus niger)
Alias
GLB; β-gal
Species
Aspergillus niger
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.23
Bio-activity
> 200 U/mg
Concentration
4,000 U/ml
Purification
Single band on isoelectric focusing.
pH Stability
3.0-8.0
Optimum pH
4.5
Optimum temperature
60°C
Thermal stability
< 70°C
Stability
> 4 years at 4°C.
Preparation Instructions
For the assay, enzyme preparation is diluted in 0.1M sodium acetate buffer pH 4.5 containing BSA (0.5 mg/mL). If BSA is excluded from the buffer lower activities are obtained.
Applications
High purity beta-Galactosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
β-galactosidase; beta-gal; β-gal; EC 3.2.1.23; lactase; β-lactosidase; maxilact; hydrolact; β-D-lactosidase; S 2107; lactozym; trilactase; β-D-galactanase; oryzatym; sumiklat; β-D-galactoside galactohydrolase
