Cat No.
NATE-0986
Description
β-galactosidase, also called beta-gal or β-gal, is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins.
Abbr
GLB, Recombinant (E. coli)
Alias
GLB
Species
E. coli
Bio-activity
> 700 U/mg protein
Appearance
White lyophilizate, stabilized with phosphate buffer and sucrose.
Molecular Mass
465 kDa
pH Stability
6
Michaelis Constant
Tris buffer, pH 7.6, +20°C / relation rate:
2-nitrophenyl-β-galactoside: 9.50 x 10-4 mol/l / 1.00
phenyl-β-D-galactoside: 3.23 x 10-3 mol/l / 0.05
lactose: 3.85 x 10-2 mol/l / 0.06
4-nitrophenyl-β-galactoside: 4.45 x 10-4 mol/l / ~0.50
Structure
4 identical subunits, β-galactosidase contains no carbohydrates
Isoelectric point
4.61
Optimum pH
8
Activators
Mg2+ and Na+ (or other monovalent cations) are essential for activity.
Thermal stability
Up to +37°C
Stability
At -15 to -25°C within specification range for 24 months. Store under nitrogen.
Applications
Marker enzyme for the manufacturing of antibody- and antigen-enzyme conjugates incorporated in immunoassays for colorimetric and fluorimetric detection.
Synonyms
β-galactosidase; beta-gal; β-gal; lactase; β-lactosidase; maxilact; hydrolact; β-D-lactosidase; trilactase; β-D-galactanase; β-D-galactoside galactohydrolase; β-Galactosidase EIA Grade
