Cat No.
NATE-0973
Description
Hydrolyzes non-reducing terminal galactose β(1-3) and β(1-4) linkages. Can be used in conjunction with other β-galactosidases for exoglycosidase sequencing.
Abbr
GLB, Native (Bovine)
Alias
β-gal; GLB
Source
Bovine testis
Species
Bovine
Form
20 mM sodium citrate phosphate, 150 mM NaCl (pH 4.0)
Molecular Mass
~68 kD
Unit Definition
One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of pNP-β-D-galactopyranoside per minute at pH 4.0 and 37°C.
Optimum pH
4
Applications
The enzyme has applications in the analysis of a wide variety of glycoconjugates. It is particularly useful for ensuring the complete removal of β(1-3) and β(1-4)-linked non-reducing terminal galactose residues of oligosaccharides. Gal β(1-6) GlcNAc is hydrolyzed more slowly, however this linkage is not normally encountered in native complex glycans. This activity towards β(1-3) and β(1-4)-linked galactose contrasts with that of our other β-galactosidases which exhibit a preference for Gal β(1-4), and cleave the Gal β(1-3) linkage relatively slowly, if at all. Used in conjunction, these enzymes provide a powerful means to determine linkage positions of non-reducing β galactose residues.
Synonyms
β-galactosidase; beta-gal; β-gal; lactase; β-lactosidase; maxilact; hydrolact; β-D-lactosidase; lactozym; trilactase; β-D-galactanase; oryzatym; sumiklat; β-D-galactoside galactohydrolase
