Cat No.
NATE-0767
Description
β-glucanases degrade β-1,4-glucans of cellulose, xyloglucan and β-1,4-xylan. β-Glucanase represents a group of carbohydrate enzymes which break down glycosidic bonds within beta-glucan. It forms the main constituent of fungal cell walls and could be a potential structural and storage polysaccharide of marine macro-algae. It has the ability to degrade fungal cell walls and may be involved in defense mechanism of plants against pathogenic fungi.
Abbr
β-Glucanase, Native (Clostridium thermocellum)
Alias
β-Glucanase
Species
Clostridium thermocellum
Enzyme Commission Number
EC 3.2.1.6
Bio-activity
314 U/mg protein (on barley β-glucan) at pH 6.5 and 40°C; 494 U/mg protein (on barley β-glucan) at pH 6.5 and 60°C
Molecular Mass
29300
pH Stability
4.0-9.0 (> 75% control activity after 24 hours at 4°C)
Isoelectric point
4.9
Unit Definition
One Unit of glucanase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from barley β-glucan (5 mg/mL) in sodium phosphate buffer (100 mM) pH 6.5.
Optimum pH
6.5
Optimum temperature
60°C
Thermal stability
up to 50°C (> 90% control activity after 15 min.)
Synonyms
endo-1,3-β-D-glucanase; laminarinase; laminaranase; β-1,3-glucanase; β-1,3-1,4-glucanase; endo-1,3-β-glucanase; endo-β-1,3 (4)-glucanase; endo-β-1,3-1,4-glucanase; endo-β-(1→3)-D-glucanase; endo-1,3-1,4-β-D-glucanase; endo-β-(1-3)-D-glucanase; endo-β-1,3-glucanase IV; endo-1,3-β-D-glucanase; 1,3-(1,3; 1,4)-β-D-glucan 3 (4)-glucanohydrolase; EC 3.2.1.6
