Cat No.
NATE-1091
Description
Beta-glucosidase is a glucosidase enzyme that acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is one of the cellulases, enzymes involved in the decomposition of cellulose and related polysaccharides; more specifically, an exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose.
Abbr
β-Glucosidase, Recombinant (Thermotoga maritima)
Species
Thermotoga maritima
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.21
Bio-activity
73.8 U/mg protein (on p-NP-β-D-Glucopyranoside) at pH 6.5 and 40°C.
Molecular Mass
~ 53,700
pH Stability
4.0-9.0
Isoelectric point
~ 6.0
Unit Definition
One Unit of β-glucosidase activity is defined as the amount of enzyme required to release one μmole of of p-nitrophenol (p-NP) per minute from p-nitrophenyl-β-D-glucopyranoside (10 mM) in sodium maleate buffer (50 mM), pH 6.5 at 40°C.
Optimum pH
7
Optimum temperature
90°C
Thermal stability
Unstable above 90°C
Stability
> 4 years at 4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium maleate buffer (50 mM), pH 6.5 containing 0.5 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity beta-Glucosidase (Thermostable) (T. maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
EC 3.2.1.21; gentiobiase; cellobiase; emulsin; elaterase; aryl-beta-glucosidase; beta-D-glucosidase; beta-glucoside glucohydrolase; arbutinase; amygdalinase; p-nitrophenyl beta-glucosidase; primeverosidase; amygdalase; linamarase; salicilinase; beta-1,6-glucosidase
