Cat No.
NATE-0777
Description
β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.
Abbr
β-Lactamase, Recombinant (Pseudomonas aeruginosa)
Alias
β-lactamase
Source
E. coli
Species
Pseudomonas aeruginosa
Form
Lyophilized powder containing sodium chloride and potassium phosphate.
Enzyme Commission Number
EC 3.5.2.6
Bio-activity
> 20 U/mg (with cephalosporin C); > 400 U/mg (with benzylpenicilin)
CAS No.
9073-60-3
Unit Definition
One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in HEPES buffer, pH 7.0 containing 10 μM zinc chloride at 25oC.
Applications
β-lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β-lactamase is used to study antibiotic resistance and resistance suppression. Product is produced from Pseudomonas aeruginosa and is expressed in E. coli.
Synonyms
β-lactamase; penicillinase; cephalosporinase; neutrapen; penicillin β-lactamase; exopenicillinase; ampicillinase; penicillin amido-β-lactamhydrolase; penicillinase I, II; β-lactamase I-III; β-lactamase A, B, C; β-lactamase AME I; cephalosporin-β-lactamase; EC 3.5.2.6; 9073-60-3
