Cat No.
NATE-1185
Description
Beta-mannosidase is an enzyme with system name beta-D-mannoside mannohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement.
Abbr
β-Mannosidase, Recombinant (Bacteroides fragilis)
Source
Bacteroides fragilis NCTC 9343
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.25
Bio-activity
5.32 U/mg
Concentration
48.05 U/ml
Molecular Mass
100520.1 Da
Purification
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of pNP per minute from pNP-β-D-mannopyranoside (1.11 mM) in 55.6 mM sodium phosphate buffer, pH 6.0, at 25 °C, and using an extinction coefficient of 18000 M-1cm-1 at 410nm.
Optimum pH
6
Synonyms
β-mannosidase; mannanase; mannase; β-D-mannosidase; β-mannoside mannohydrolase; exo-β-D-mannanase; EC 3.2.1.25; 9025-43-8
