Cat No.
NATE-0782
Description
Hexosaminidase, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.
Abbr
β-N-Acetylglucosaminidase, Recombinant (Streptococcus pneumoniae)
Source
E. coli
Species
Streptococcus pneumoniae
Form
buffered aqueous solution, Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl
Enzyme Commission Number
3.2.1.52
Bio-activity
> 80 units/mg protein
CAS No.
9012-33-3
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at the pH 5 at 37°C.
Package
vial of > 1.0 unit
Applications
Hexosaminidase is an enzyme involved in the hydrolysis of terminal N-acetyl-D-hexosamine residues in N-acetyl-β-D-hexosaminides.
Gene Name
lytB endo-beta-N-acetylglucosaminidase [Streptococcus pneumoniae R6]
Synonyms
EC 3.2.1.52; 9012-33-3; hexosaminidase; β-acetylaminodeoxyhexosidase; N-acetyl-β-D-hexosaminidase; N-acetyl-beta-hexosaminidase; β-hexosaminidase; β-acetylhexosaminidinase; β-D-N-acetylhexosaminidase; β-N-acetyl-D-hexosaminidase; β-N-acetylglucosaminidase; hexosaminidase A; N-acetylhexosaminidase; β-D-hexosaminidase
GeneID
Streptococcus pneumoniae R6 ... lytB (934406)
