Cat No.
NATE-0781
Description
This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.
Abbr
β-N-Acetylglucosaminidase, Native (Human)
Source
Human placenta
Species
Human
Form
ammonium sulfate suspension. Suspension in 2.4 M (NH4)2SO4 containing 0.15 M NaCl and 0.1 M sodium phosphate, pH 6.0
Enzyme Commission Number
EC 3.2.1.52
Bio-activity
6-20 units/mg protein
CAS No.
9012-33-3
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at the pH 4.25 at 25°C.
Applications
β-N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer’s Disease.
Gene Name
ENGASE endo-beta-N-acetylglucosaminidase [Homo sapiens]; HEXA hexosaminidase A (alpha polypeptide) [Homo sapiens]
Synonyms
hexosaminidase; β-acetylaminodeoxyhexosidase; N-acetyl-β-D-hexosaminidase; N-acetyl-beta-hexosaminidase; β-hexosaminidase; β-acetylhexosaminidinase; β-D-N-acetylhexosaminidase; β-N-acetyl-D-hexosaminidase; β-N-acetylglucosaminidase; hexosaminidase A; N-acetylhexosaminidase; β-D-hexosaminidase; 9012-33-3; EC 3.2.1.52
GeneID
64772; 3073
mRNA Refseq
NM_001042573.2; NM_000520
Protein Refseq
NP_001036038.1; NP_000511
MIM
611898; 606869
UniProt ID
Q8NFI3; P06865
Chromosome Location
17q25.3; 15q24.1
Pathway
Other glycan degradation, organism-specific biosystem;Other glycan degradation, conserved biosystem; Amino sugar and nucleotide sugar metabolism, organism-specific biosystem; Amino sugar and nucleotide sugar metabolism, conserved biosystem; Glycosaminoglycan degradation, organism-specific biosystem; Glycosaminoglycan degradation, conserved biosystem; Glycosphingolipid biosynthesis-ganglio series, organism-specific biosystem; Glycosphingolipid biosynthesis-ganglio series, conserved biosystem; Glycosphingolipid biosynthesis-globo series, organism-specific biosystem
Function
mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity; beta-N-acetylhexosaminidase activity; cation binding; hydrolase activity, hydrolyzing O-glycosyl compounds; protein heterodimerization activity
