Cat No.
NATE-1044
Description
This enzyme releases non-reducing terminal β1-2, β1-3, β1-4 and β1-6 linked N-acetylglucosamine from complex carbohydrates. When incubated with oligosaccharides at low concentrations (<50 mU/ml) the enzyme can differentiate between GlcNAcβ1-2Man, GlcNAcβ1-4Man and GlcNAcβ1-6Man linkages. Under such conditions, the enzyme cleaves essentially only β1-2 linked GlcNAc, with two provisos. Firstly, β1-2 GlcNAc is not hydrolyzed if the mannose to which it is substituted has a substitution at C-6. Thus, the enzyme is useful for the analysis of tri-antennary oligosaccharides. Secondly, if the β-linked mannose of the conserved pentasaccharide core is substituted with a "bisecting" GlcNAc then only the β1-2 linked GlcNAc linked to mannose on the α1-3 arm is cleaved. At higher concentrations of the enzyme, β1-4 and β1-6 linked GlcNAc may also be hydrolyzed.
Abbr
β-N-Acetylhexosaminidase, Recombinant (Microbial)
Species
Microbial
Form
Lyophilized powder
Enzyme Commission Number
EC 3.2.1.52
Bio-activity
1.4 U/mg protein (on pNP-β-D-N-acetylglucosamine) at pH 7.5 and 37°C.
Molecular Mass
~ 52,400
Isoelectric point
~ 5.7
Specificity
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-β-Dhexosaminides from glycoproteins and oligosaccharides.
Unit Definition
One Unit of β-N-acetyl-D-hexosaminidase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from pNP-β-D-Nacetylglucosamine (1 mM) in TRIS.HCl buffer (100 mM) pH 7.5 and 37°C, monitored at 410 nm.
Optimum pH
6.0 - 8.0
Stability
Lyophilised powder: > 4 years at -20°C
Reconstituted enzyme: > 6 months at 4°C or > 2 years at -20°C
Preparation Instructions
For assay, this enzyme should be diluted appropriately in distilled water immediately prior to use.
Applications
High purity recombinant beta-N-Acetylhexosaminidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase; β-N-Acetylhexosaminidase; N-Acetyl-β-D-glucosaminidase, β-N-Acetylglucosaminidase
