Cat No.
NATE-1133
Description
Releases reducing sugars from birchwood xylan (X0502), also catalyzes the hydrolysis of 4-methylumbelliferyl-β-D-cellobioside and 4-methylumbelliferyl-β-D-glucopyranoside. This enzyme does not possess endo-xylanase, arabinoxylanase or β-glucanase activities. β-Xylosidase undergoes post-translation glycosylation which has been shown to be critical for its proper activity and stability. Deglycosylation altered the the optimum temperature and pH for activity and decreased its thermostability.
Abbr
β-Xylosidase, Recombinant (B. pumilus)
Species
Bacillus pumilus
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.37
Bio-activity
18.2 U/mg protein at pH 7.5 and 35°C; ~ 40 U/mg protein at pH 7.5 and 35°C on xylobiose.
Molecular Mass
~ 61,190
Isoelectric point
~ 5.7
Unit Definition
One Unit of β-xylosidase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol (p-NP) per minute from p-nitrophenyl-β-Dxylopyranoside (5 mM) in potassium phosphate buffer (50 mM), pH 7.5 at 35°C.
Optimum pH
7.5
Optimum temperature
35°C
Preparation Instructions
For assay, this enzyme should be diluted in potassium phosphate buffer (50 mM), pH 7.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant beta-Xylosidase (B. pumilus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
β-Xylosidase; EC 3.2.1.37; exo-1,4-β-D-xylosidase; 1,4-β-D-xylan xylohydrolase; β-D-Xylosidase
