Cat No.
NATE-0799
Description
In enzymology, an acetylxylan esterase (EC 3.1.1.72) is an enzyme that catalyzes a chemical reaction, the deacetylation of xylans and xylo-oligosaccharides. This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds.
Abbr
Acetylxylan esterase, Recombinant (Orpinomyces sp.)
Species
Orpinomyces sp.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.1.1.72
Bio-activity
200 U/mg protein (on 4-nitrophenyl acetate) at pH 6.7 and 40°C.
CAS No.
188959-24-2/9000-82-2
Molecular Mass
~ 34,000 Da
pH Stability
3.0 - 9.0 (> 75% control activity after 24 hours at 4°C)
Isoelectric point
~ 5.6
Specificity
Catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, α-napthyl acetate and 4-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin.
Unit Definition
One Unit of acetylxylan esterase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from 4-nitrophenyl acetate in sodium phosphate buffer (100 mM), pH 6.7 at 40°C.
Optimum pH
7
Optimum temperature
40°C
Stability
> 2 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 6.7 containing 1.0 mg/ml BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant Acetylxylan esterase (Orpinomyces sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
Acetylxylan esterase; EC 3.1.1.72; 188959-24-2; 9000-82-2
