Cat No.
NATE-0089
Description
ATPases are a class of enzymes that catalyze the decomposition of ATP into ADP and a free phosphate ion. This dephosphorylation reaction releases energy, which the enzyme (in most cases) harnesses to drive other chemical reactions that would not otherwise occur. This process is widely used in all known forms of life. Some such enzymes are integral membrane proteins (anchored within biological membranes), and move solutes across the membrane, typically against their concentRation gradient. These are called transmembrane ATPases.
Abbr
ATPase, Native (Porcine)
Alias
ATPase
Source
Porcine cerebral cortex
Species
Porcine
Form
Lyophilized powder containing 90% sucrose, 0.4% EDTA Na4 and 0.06% NaCl
Enzyme Commission Number
EC 3.6.1.3
Bio-activity
> 0.3 units/mg protein
CAS No.
9000-83-3
Unit Definition
One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37°C in the presence of Na+, K+, and Mg2+.
Applications
ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity.
Gene Name
ATP1A1 ATPase, Na+/K+ transporting, alpha 1 polypeptide [Sus scrofa]; ATP1B1 ATPase, Na+/K+ transporting, beta 1 polypeptide [Sus scrofa]
Synonyms
ATP phosphohydrolase; ATPase; Adenosine 5'-Triphosphatase; EC 3.6.1.3; adenylpyrophosphatase; ATP monophosphatase; triphosphatase; SV40 T-antigen; adenosine 5'-triphosphatase; ATP hydrolase, complex V (mitochondrial electron transport); (Ca2+ + Mg2+)-ATPase; HCO3?-ATPase; adenosine triphosphatase
GeneID
397481; 396898
mRNA Refseq
NM_214249; NM_001001542
Protein Refseq
NP_999414; NP_001001542
UniProt ID
P05024; P05027
Chromosome Location
4; 4q1.6-q2.3; 4; 4q1.3-q2.1
Pathway
Adrenergic signaling in cardiomyocytes, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Cardiac muscle contraction, conserved biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Cardiac muscle contraction, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, conserved biosystem
Function
ATP binding; potassium ion binding; sodium ion binding; ATPase activator activity; protein binding; sodium:potassium-exchanging ATPase activity
