Cat No.
NATE-1293
Description
Agarase is an enzyme with system name agarose 4-glycanohydrolase. It found in agarolytic bacteria and is the first enzyme in the agar catabolic pathway. It is responsible for allowing them to use agar as their primary source of Carbon and enables their ability to thrive in the ocean. Agarases are classified as either α-agarases or β-agarases based upon whether they degrade αor β linkages in agarose, breaking them into oligosaccharides. When secreted, α-agarases yield oligosaccharides with 3.6 anhydro-L-galactose at the reducing end whereas β-agarases result in D-galactose residues.
Abbr
β-Agarase 16D, Recombinant (Zobellia galactanivorans)
Source
E. coli
Species
Zobellia galactanivorans
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.81
Concentration
0.25 mg/mL
Molecular Mass
42.4 kDa
Purification
>90% by SDS-PAGE
Specificity
Agarose
Optimum pH
7
Optimum temperature
20-30 °C
Synonyms
agarase; AgaA; AgaB; endo-β-agarase; agarose 3-glycanohydrolase; EC 3.2.1.81; 37288-57-6
