Cat No.
NATE-0055
Description
Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
Abbr
ALP, Native (Chicken)
Alias
ALP; ALKP
Source
Chicken Intestine.
Species
Chicken
Form
dried powder
Enzyme Commission Number
EC 3.1.3.1
Bio-activity
> 0.9 units per mg dry weight (25°C pH 8.8
Molecular Mass
140 kDa
Purification
Partially purified
Composition
The enzyme is a zinc metallo-enzyme. Schüssler (1968) reports four isozymes. Chang and Moog (1972) found three isozymes in the enzyme from chicken duodenum.
Isoelectric point
5.7.
Unit Definition
One Unit hydrolyzes 1μmole of o-carboxyphenol phosphate per minute at 25°C, pH 8.8.
Optimum pH
42225
Activators
Schüssler (1968) indicates activation by Mg2+. See Sivanaesan et al. (1991).
Stability
The lyophilized preparation is stable for 1-2 years at 2-8°C.
Inhibitors
Acidification to pH 4.5 reversibly inactivates the enzyme.
Gene Name
ALPI alkaline phosphatase, intestinal [Gallus gallus]
Synonyms
Alkaline phosphatase; ALP; ALKP; ALPase; Alk Phos; EC 3.1.3.1; Alkaline phosphomonoesterase; Glycerophosphatase; Phosphomonoesterase
GeneID
424936
Chromosome Location
chromosome: 9
Pathway
Folate biosynthesis, organism-specific biosystem; Folate biosynthesis, conserved biosystem; Metabolic pathways, organism-specific biosystem
