Cat No.
NATE-0056
Description
Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
Abbr
ALP, Native (E. coli)
Alias
ALP; ALKP
Species
Escherichia coli
Form
A suspension in 2.6M ammonium sulfate, pH 8.0.
Enzyme Commission Number
EC 3.1.3.1
Bio-activity
Type I, >30 units per mg protein; Type II, >20 units per mg protein; Type III, >10 units per mg protein.
CAS No.
9001-78-9
Unit Definition
One Unit hydrolyzes 1μmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.
Applications
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and hist ochemical detection. It may be used for protein labeling when high sensitivity is required.
Warnings
Protein determined by biuret.
Synonyms
Alkaline phosphatase; ALP; ALKP; ALPase; Alk Phos; EC 3.1.3.1; Alkaline phosphomonoesterase; Glycerophosphatase; Phosphomonoesterase
