Cat No.
NATE-0051
Description
Alpha-lytic protease (aLP) is an alternative specificity protease for proteomics applications, whose wild-type (WT) version cleaves after T, A, S, and V residues. The M190A (Met190 → Ala190) mutant of aLP has different cleavage specificities, and cleaves after M, F, and L residues. Both the WT and M190A forms of aLP geneRate peptides of similar average length as trypsin.
Abbr
ALP M190A, Recombinant
Alias
ALP M190A
Bio-activity
> 0.05 U/mg
Isoelectric point
9.69
Unit Definition
mmols of pNA produced per min per mg protein from 0.5 mM N-succinyl-Ala-Ala-Pro-Phe-PNA at 25°C in 100 mM Tris-HCl (pH 7.5).
Optimum pH
5.0(storage); 7.5(activity)
Synonyms
ALP M190A; Alpha-Lytic Protease M190A Mutant
