Cat No.
NATE-1298
Description
Glucan 1,4-alpha-maltohydrolase (EC 3.2.1.133, maltogenic alpha-amylase, 1,4-alpha-D-glucan alpha-maltohydrolase) is an enzyme with system name 4-alpha-D-glucan alpha-maltohydrolase. This enzyme catalyses the following chemical reaction:hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive alpha-maltose residues from the non-reducing ends of the chains. This enzyme acts on starch and related polysaccharides and oligosaccharides.
Abbr
Maltogenic α-amylase 13A, Recombinant (Lactobacillus gasseri)
Source
E. coli
Species
Lactobacillus gasseri
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.133
Concentration
0.25 mg/mL
Molecular Mass
55.0 kDa
Purification
>90% by SDS-PAGE
Specificity
β-Cyclodextrin (β-CD), starch and pullulan, thus producing maltose from β-CD and starch, and panose from pullulan. The enzyme activity is strongly inhibited by Zn2+, Fe2+, Co2+ and EDTA
Optimum pH
5
Optimum temperature
55 °C
Synonyms
Glucan 1,4-alpha-maltohydrolase; EC 3.2.1.133; maltogenic alpha-amylase; 1,4-alpha-D-glucan alpha-maltohydrolase; Glucan 1,4-α-maltohydrolase, Maltogenic Amylase, Novamyl 1000BG
