Cat No.
NATE-1079
Description
Glucan 1,4-alpha-glucosidase is an enzyme located on the brush border of the small intestine with system name 4-alpha-D-glucan glucohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis0 of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose. Most forms of the enzyme can rapidly hydrolyse 1,6-alpha-D-glucosidic bonds when the next bond in the sequence is 1,4.
Abbr
Amyloglucosidase, Recombinant (Hormoconis resinae)
Species
Hormoconis resinae
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.3
Bio-activity
159 U/mg protein (on soluble starch) at pH 4.5 and 60°C; 64 U/mg protein (on soluble starch) at pH 4.5 and 40°C.
Molecular Mass
~ 65,400
pH Stability
3.0 - 9.0
Isoelectric point
~ 4.9
Specificity
Hydrolysis of terminal non-reducing α-1,4-D-glycosidic bonds in α-1,4-D-glucans with "debranching activity" (hydrolysis of α-1,6-D-glycosidic bonds) in substrates such as starch and pullulan.
Unit Definition
One Unit of glucoamylase activity is defined as the amount of enzyme required to release one μg of β-D-glucose reducing-sugar equivalents per minute from soluble starch(10 mg/mL) in sodium acetate buffer (100 mM) at pH 4.5.
Optimum pH
4.0 - 5.0
Optimum temperature
60°C
Thermal stability
up to 50°C
Stability
> 4 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 4.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant Amyloglucosidase (Glucoamylase P - H. Resinae) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
glucoamylase; amyloglucosidase; γ-amylase; lysosomal α-glucosidase; acid maltase; exo-1,4-α-glucosidase; glucose amylase; γ-1,4-glucan glucohydrolase; acid maltase; 1,4-α-D-glucan glucohydrolase; EC 3.2.1.3; 9032-08-0
