Cat No.
NATE-1051
Description
Glucan 1,4-alpha-glucosidase is an enzyme located on the brush border of the small intestine with system name 4-alpha-D-glucan glucohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis0 of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose. Most forms of the enzyme can rapidly hydrolyse 1,6-alpha-D-glucosidic bonds when the next bond in the sequence is 1,4.
Abbr
Amyloglucosidase, Native (Aspergillus niger) (Glycerol Free)
Species
Aspergillus niger
Form
Buffered solution
Enzyme Commission Number
EC 3.2.1.3
Bio-activity
~ 6520 U/mL (40°C, pH 4.5 on soluble starch)
CAS No.
9032-08-0
Molecular Mass
~ 143,500
pH Stability
4.0-5.5
Isoelectric point
~ 4.0
Specificity
Hydrolysis of terminal (1,4)-linked a-D-glucose residues successively from non-reducing ends of the chains with release of b-D-glucose.
Unit Definition
One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) at pH 4.5 and 40°C
Optimum pH
4
Optimum temperature
70°C
Thermal stability
< 60°C
Stability
> 4 years at 4°C.
Preparation Instructions
The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard .
The preparation is effectively devoid of cellulase and is free of catalase.
Applications
This enzyme is recommended for use in Total Dietary Fibre analytical procedures and the Total Starch test method.
Synonyms
glucoamylase; amyloglucosidase; γ-amylase; lysosomal α-glucosidase; acid maltase; exo-1,4-α-glucosidase; glucose amylase; γ-1,4-glucan glucohydrolase; acid maltase; 1,4-α-D-glucan glucohydrolase; EC 3.2.1.3; 9032-08-0; glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase
