Cat No.
NATE-1399
Description
Antarctic Phosphatase catalyzes the removal of 5′ phosphate from DNA and RNA. Since phosphatase-treated fragments lack the 5′ phosphoryl termini required by ligases, they cannot self-ligate. This property can be used to decrease the vector background in cloning strategies.
Abbr
Antarctic Phosphatase, Recombinant (E. coli)
Source
E. coli
Species
E. coli
Form
10 mM Tris-HCl (pH 7.4), 1 mM MgCl2, 0.01 mM ZnCl2, 1 mM DTT and 50% glycerol.
Concentration
5,000 units/ml
Molecular Mass
Apparent: 35 kDa
Theoretical: 69 kDa
Purification
>95% estimated by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme that will dephosphorylate 1 μg of pUC19 vector DNA cut with HindIII (5′ protruding ends), HincII (blunts ends) or PstI (5′ recessed ends) in 30 minutes at 37°C. Dephosphorylation is defined as > 95% inhibition of recirculation in a self-ligation reaction and is measured by transformation into E. coli.
Applications
Removing 5′ phosphates from DNA, RNA, rNTPs and dNTPs
Preparation of templates for 5′ end labeling
Prevention of recircularization of cloning vectors
Removal of dNTPs and pyrophosphate from PCR reactions
Dephosphorylation of proteins
Synonyms
Antarctic Phosphatase
